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2016-6-14

A group of researchers from Kyushu University, System in Frontier Inc., Osaka University, and University of Tsukuba, as a part of Japan Science and Technology Agency’s Development of Systems and Technology for Advanced Measurement and Analysis, developed a prototype of an “in situ,” or “real time” electron-beam tomography system, which is capable of shooting  3D images of structural changes resulting from heating and straining by using a Transmission Electron Microscope (TEM) capable of observing nanoscale cells and materials

In May 2016, using this system, in cooperation with Mel-Build Company and Virginia Polytechnic Institute and State University, this group succeeded in shooting real time 3D images of the plastic deformation process of nanoscale metal, which was deformed by force, verifying the effectiveness of this system. This is the first 3D imaging of irreversible plastic deformation in TEM.

In this real time ET system, various technologies are used:

  1. 1. A Straining-And-Tomography (SATO) holder capable of holding a specimen to a tilt angle of 60 degrees or more while deforming it in TEM at a nanoscale level. Ultra-small deformation with a minimum amount of displacement of 1nm is available within a 10-4~10-6/sec of strain rate.
  2. 2. Integrated control application software, which can automatically carry out two things: (1) straining and high-angle tilt by a SATO holder in TEM, and (2) recording of continuous tilting images necessary for reconstructing a frame of 3D image within three minutes. This has enabled continuous recording of 20 frames or more of 3D images of the nanoscale specimen deformation process every three minutes.
  3. 3. TEMographyTM software for reconstructing 3D still images and videos connecting these 3D still images. Thanks to the development of a new algorithm using compressed sensing, Iterative Series Reduction (ISER), it has become possible to reconstruct a 3D structure of a real object from even a small number of continuous tilting or noisy images.

Imaging technology developed by this group has enabled observation of 3D imaging of real time nanoscale structural changes in deformation by force in TEM, so it is expected to be used in fields where nanostructure analysis is in great demand.

This group’s achievement was presented at the 72nd academic lectures of The Japanese Society of Microscopy, which was held at Sendai International Center from Tuesday, June 14 through Thursday, June 16, 2016.

Abstract

We have developed a newly designed straining specimen holder for in situ transmission electron microscopy (TEM) compatible with high-angle single tilt-axis electron tomography. The holder can deform a TEM specimen under tensile stress with the strain rate between 1.5 × 10−6 and 5.2 × 10−3 s−1. We have also confirmed that the maximum tilt angle of the specimen holder reaches ±60° with a rectangular shape aluminum specimen. The new specimen holder, termed as ‘straining and tomography holder’, will have wide range potential applications in materials science.

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Real time ET system (movie)

To learn more about this research, please view the full research report entitled “Development of a novel straining holder for transmission electron microscopy compatible with single tilt-axis electron tomography” at this page of the Microscopy website.


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